pMTL80000 modular plasmids
The pMTL80000 plasmids are modular shuttle vectors for use in Clostridia and other anaerobes. They essentially comprise four modular parts that are combined through four, unique restriction enzymes with 8bp recognition sequences: AscI, FseI, PmeI and SfbI.
Parts comprise a Gram +ve and –ve replicon, selectable marker, and application specific module. A fifth module comprising oriT/traJ may be added as a unique ApaI fragment should mobilisation by conjugative plasmid transfer be required. Parts are arranged relative to one another in the same order and orientation. Each exists as at least two variants – with a total of 18 parts, 395 different vectors were possible when originally published:–
These are distributed as five plasmids, which together contain all 18 modules, by the Synthetic Biology Research Centre (SBRC) at the University of Nottingham. Since publication, the number of modules has been expanded to include 5 new, Gram +ve replicons and a part encoding the FAST reporter protein.
Details on availability, vector nomenclature and where to source the plasmids and sequences may be found at www.plasmidvectors.com.